文章编号：1004－5422(2022)01－0006－06

DOI:10.3969/j.issn.1004-5422.2022.01.002

基于酶活性丹参茎叶散剂活血活性谱—效模型研究

杨　慧¹，张　佳²，钟林江³，戴　静³，郭晓恒^4，5

(1.江西中医药大学现代中药制剂教育部重点实验室，江西南昌330004;

2.成都大学食品与生物工程学院，四川成都610106;

3.成都大学四川抗菌素工业研究所，四川成都610052;4.成都大学基础医学院，四川成都610106;

5.四川省抗病毒中药产业化工程技术研究中心，四川成都610106)

摘　要：采用HPLC法和纤维蛋白原平板法，测定6批丹参茎叶散剂的特征图谱和体外活血活性值，并对特征图谱的特征峰进行指认，分析其特征峰成分与活血活性的相关性；采用SPSS软件，经主成分分析、相关性分析和多重线性回归分析的方法构建其谱—效模型，并用6批丹参茎叶散剂对其进行验证.丹参茎叶散剂特征图谱中得到9个特征峰，体外活血活性值在132.52～153.25 U/mL.根据主成分分析，综合解释总方差和碎石图分析，得该模型的最优主成分数为5，由Spearman相关性分析得到5个主成分色谱峰和活血活性相关性均大于0.657，且对活血作用显示正相关，预测值和测量值的相对误差较小.建立的基于酶活性的活血活性谱—效模型可较好地预测丹参茎叶散剂的活血活性，为其进一步开发利用提供基础.

关键词：丹参茎叶散剂；特征图谱；纤维蛋白原平板；谱—效关系

中图分类号：R284.1;O657.72    文献标志码：A

Study on Activity Spectrum Effect Model of Stem and Leaf-Powder of Salvia Miltiorrhiza Based on Enzyme Activity

YANG Hui¹,ZHANG Jia²,ZHONG Lingjiang³,DAI Jing³,GUO Xiaoheng^4，5

（1.Key Laboratory of Modern Chinese Medicine Preparations of Ministry of Education,Jiangxi University of Traditional Chinese  Medicine,Nanchang 330004,China;2.College of Food and Biological Engineering,Chengdu University,Chengdu 610106,China;3.Sichuan Industrial Institute of Antibiotics,Chengdu University,Chengdu 610052,China;4.School of Preclinical Medicine,Chengdu University,Chengdu 610106,China;5.Sichuan Engineering Technology Research Center for Antiviral Traditional Chinese Medicine Industrialization,Chengdu 610106,Sichuan)

Abstract：Six batches of  Salvia miltiorrhiza stem and leaf powder were determined by HPLC and fibrinogen plate method.The feature map and in vitro activating activity value of Salvia miltiorrhiza stem and leaf powder were measured.The characteristic peak of the feature map was identified by reference substance,and the correlation between the component of the characteristic peak and its activating activity was analyzed.By using the SPSS software,the spectral efficiency model was established by principal component analysis,correlation analysis and multiple linear regression analysis method,and 6 batches of  Salvia miltiorrhiza stem and leaf powder were used for verification.The results showed that nine characteristic peaks were obtained in the feature map of stem and leaf powder of  Salvia miltiorrhiza.The activating blood activity value in vitro was 132.52 ~ 153.25 U/mL.According to principal component analysis,comprehensive interpretation of total variance and gravel diagram analysis,the optimalprincipal component number of this model was 5.Spearman correlation analysis showed that the correlation between the five principal component chromatographic peaks and the activating blood activity was all greater than 0.657,and showed a positive correlation with the activating blood activity.The relative error between the predicted value and the measured value was small.It is concluded that the active blood activity spectrum effect model based on enzyme activity can predict the active blood activity of the stem and leaf powder of Salvia miltiorrhiza,and provide the basis for its clinical application.

Key words：Salvia miltiorrhiza;feature map;fibrinogen tablets;spectral validity