魏玉娇1， 郭晓强1， 杨淞瑞2， 李小红1， 何源超2， 何顺东2， 刘达玉1
Technical Study on Extraction of Astilbin from Smilacis Glabra Rhizoma by
Response Surface Method
Abstract:By using Smilacis glabra Rhizoma as the raw material,ultrasonic method was used to extract astilbin.High performance liquid chromatography(HPLC) was used for the quantitative determination of astilbin in the extract of Smilacis glabra Rhizoma.Based on the single-factor experiment,the BOX-Behnken response surface method was used to investigate the effects of ethanol concentration,solidliquid ratio and ultrasonic time on the content of astilbin in the extraction liquid of Smilacis glabra Rhizoma,in order to determine the optimal extraction process of astilbin from Smilacis glabra Rhizoma.The results showed that when the concentration of ethanol was 67.7%,the extraction time was 875 min,and the ratio of material to liquid was 1∶10.3 g:mL,the content of astilbin in the extract of Smilacis glabra Rhizoma was 0.0621 mg/mL.The experimental results show that the average deviation between the predicted value and the measured value is 2.0%,which indicates that the mathematical model established in this experiment has good predictability and the extraction process is accurate and feasible.Finally,the extraction process is determined to be:the extraction time is 90 min,the ethanol concentration is 70%,the matterliquid ratio is 1∶10 g:mL,and the average content of astilbin in the extraction solution is 0.0611 mg/mL.
Key words:Smilacis glabra Rhizoma;astilbin;high performance liquid chromatography;response surface optimization